In this explorative study, we have demonstrated the
potential of two strains, Pseudomonas KA19 and Bacillus
SE, isolated from rhizosphere soil of Brassica campestris,
in biocontrol of black rot caused by Xcc. Based on 16S
rRNA gene analysis, strains KA19 and SE were classified
as closest to P. aeruginosa and B. thuringiensis respectively. Both KA19 and SE were effective against Xcc. On
one hand, Pseudomonas KA19 was better than Bacillus SE
in controlling the disease lesions of black rot, and on the
other hand, Bacillus SE was better in plant growth promotion compared to Pseudomonas KA19. Moreover, the
co-inoculation (KA19 ? SE) provided better management
of black rot. It has been suggested earlier that by combining specific strains of microorganisms with multiple
traits antagonizing the pathogen, a higher level of protection can be achieved (Spadaro and Gullino 2005).
The data suggested that biocontrol agents have growthpromoting activities. Enhanced plant growth in addition to
disease control has been achieved with Pseudomonas and
Bacillus (Whipps 2001; Sharma et al. 2010). Biocontrol of
minor pathogens in the rhizosphere, improved nutrient
release, mineral uptake from soil and organic matter and
enhanced plant hormone production may partially explain
the biocontrol mediated growth response (Dumas-Gaudot
et al. 1984). Siderophores have been shown to play a role in
increased growth response of certain plants along with
reduction of plant pathogen (Whipps 2001). There was an
increase in the dry matter content, lipids, and proteins in
the seed with the application of KA19 and SE. This
enhancement of lipids in the seeds influenced by biocontrol
In this explorative study, we have demonstrated thepotential of two strains, Pseudomonas KA19 and BacillusSE, isolated from rhizosphere soil of Brassica campestris,in biocontrol of black rot caused by Xcc. Based on 16SrRNA gene analysis, strains KA19 and SE were classifiedas closest to P. aeruginosa and B. thuringiensis respectively. Both KA19 and SE were effective against Xcc. Onone hand, Pseudomonas KA19 was better than Bacillus SEin controlling the disease lesions of black rot, and on theother hand, Bacillus SE was better in plant growth promotion compared to Pseudomonas KA19. Moreover, theco-inoculation (KA19 ? SE) provided better managementof black rot. It has been suggested earlier that by combining specific strains of microorganisms with multipletraits antagonizing the pathogen, a higher level of protection can be achieved (Spadaro and Gullino 2005).The data suggested that biocontrol agents have growthpromoting activities. Enhanced plant growth in addition todisease control has been achieved with Pseudomonas andBacillus (Whipps 2001; Sharma et al. 2010). Biocontrol ofminor pathogens in the rhizosphere, improved nutrientrelease, mineral uptake from soil and organic matter andenhanced plant hormone production may partially explainthe biocontrol mediated growth response (Dumas-Gaudotet al. 1984). Siderophores have been shown to play a role inincreased growth response of certain plants along withreduction of plant pathogen (Whipps 2001). There was anincrease in the dry matter content, lipids, and proteins inthe seed with the application of KA19 and SE. Thisenhancement of lipids in the seeds influenced by biocontrol
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