Figure 1. Quantitative RT-PCR analysis of desaturase genes. Expression of Δ9-Δ12-Δ15 desaturase genes in control and recombinant E. coli TOP10 cells. E. coli cells were cultured in LB + stearic acid (0.2 mM) and harvested at 0 h, 4 h and 6h for qRT-PCR and GC-MS analyses.