A study was conducted at Haramaya district in east Hararghe Zone of Oromia Regional State, Ethiopia to determine the prevalence of E. coli on Ethiopia Birr (ETB) paper currency notes handled by various food type handlers with E. coli. Simple random sampling technique was applied both on the food handlers and the notes types of currency at Haramaya University (HU) and the surrounding, and at Haramaya town.