Objective: To identify the major volatile constituents and evaluate the antibacterial activity of
essential oil isolated from the aerial parts of Ocimum basilicum (O. basilicum) Linn. grown in
Oman.
Methods: The fresh plant material was collected in the month of September from Seeb
Nursery in Muscat Governorate, Sultanate of Oman. The aerial parts of O. basilicum Linn.
were separated from fresh plant and essential oil was isolated by hydrodistillation method
by using Clevenger apparatus. A greenish yellow oil was obtained in 0.6% v/w yield which
was analyzed by gas chromatography–mass spectrometry for its chemical composition. The
antibacterial activity of oil was also evaluated against three Gram–positive and four Gram–
negative pathogenic bacterial strains by agar well diffusion method.
Results: A total of thirty six chemical constituents were identified and linalool (69.87%) was
found to be the major constituent. Other main identified constituents included geraniol (9.75%),
p-allylanisole (6.02%), 1,8–cineole (4.90%), trans–琢–bergamotene (2.36%) and neryl acetate
(1.24%). The essential oil of O. basilicum showed excellent antibacterial activity against
Gram–positive bacteria and moderate activity against Gram–negative bacteria.
Conclusions: Omani basil is characterized by a high content of linalool which makes it useful
in food, pharmaceutical and perfumery industries.
Objective: To identify the major volatile constituents and evaluate the antibacterial activity ofessential oil isolated from the aerial parts of Ocimum basilicum (O. basilicum) Linn. grown inOman.Methods: The fresh plant material was collected in the month of September from SeebNursery in Muscat Governorate, Sultanate of Oman. The aerial parts of O. basilicum Linn.were separated from fresh plant and essential oil was isolated by hydrodistillation methodby using Clevenger apparatus. A greenish yellow oil was obtained in 0.6% v/w yield whichwas analyzed by gas chromatography–mass spectrometry for its chemical composition. Theantibacterial activity of oil was also evaluated against three Gram–positive and four Gram–negative pathogenic bacterial strains by agar well diffusion method.Results: A total of thirty six chemical constituents were identified and linalool (69.87%) wasfound to be the major constituent. Other main identified constituents included geraniol (9.75%),p-allylanisole (6.02%), 1,8–cineole (4.90%), trans–琢–bergamotene (2.36%) and neryl acetate(1.24%). The essential oil of O. basilicum showed excellent antibacterial activity againstGram–positive bacteria and moderate activity against Gram–negative bacteria.Conclusions: Omani basil is characterized by a high content of linalool which makes it usefulin food, pharmaceutical and perfumery industries.
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