2.2. Simulating sausage fermentation
A total amount of 18 kg minced beef meat packed in modified atmosphere
(80% O2/20% CO2) with a fat content of 16–20% was purchased
from a local shop. The average level of lactic acid bacteria in the minced
meat was determined to 3.8 (±0.7) log10 cfu g−1 on DeMan, Rogosa
and Sharpe (MRS, Oxoid CM0361) plates. The mince was inoculated
with 5.1 (±0.16) and 5.3 (±0.29) log10 cfu g−1 of stationary- and
exponential-phase S. Typhimurium cells of DTU292 and DT12,
respectively. This made up four different bacterial conditions: 1) DT12
cells in exponential phase, 2) DT12 cells in stationary phase,
3) DTU292 cells in exponential phase and 4) DTU292 cells in stationary
phase.