Fibrous structure of gelatin scaffolds was confirmed by SEM(Fig. 1).
Fibres were smooth and uniformly structured. At certain glucose content,
the average fibre diameters varied almost up to two times depending
on electrospinning conditions (voltage, distance between needle tip
and grounded collector, relative air humidity etc.). For example, the average
fibre diameters of pure gelatin scaffolds prepared using different
electrospinning parameters varied between 280 and 575 nm. Fibre diameters
decreased until glucose content reached about 5%, then started
to increase again as glucose concentration became higher. The dropping
of fibre diameters is probably associated with the effect of glucose,
whereas the increase of fibre size can be explained by the increasing
viscosity of electrospinning solution and the fact that while every
electrospinning solution contained 25% gelatin, it contained a smaller
and smaller percentage of solvent as glucose was added to the solution.