The content of chlorophyll a and b as well as of carotenoids was
analyzed spectrophotometrically by the method of Lichtenthaler
and Wellburn [39]. Total anthocyanins were determined using
the method described by Mancinelli [40]. For one replicate randomly
selected and precisely weighed (200–400 mg) tissue of
leaves or epicotyls was taken. In case of carotenoids and chlorophylls
determination, a 80% of acetone solution in a ratio 1 mg of
plant tissue to 50 ll of the solvent was used. For the extraction
of anthocyanins a 1% solution of HCl in methanol, in a ratio 1 mg
of plant tissue to 40 ll of the solvent was applied.