Preparation of extracts
The fresh leaves and stem of Wedelia chinensis were thoroughly
washed to remove debris and the earth remains. From
these the stem were divested and chopped into bits and allowed to dry for 7-14 days under shade at the room temperature.
The 300g of dried leaves and stem were weighed using
an electronic weighing balance and powdered by using electrical
grinder. The powder was soaked in hexane (1200ml),
acetone (1200ml), ethylacetate (1200ml) and methanol
(1200ml). The powder soaked in solvents for three days. The
extracts were filtered through a Buchner funnel with Whatmann’s
number 1 filter paper. Then the filtered sample was
poured into a soxhlet apparatus (boiling point range 100°C).
After 2 hours the filter paper was concentrated at room temperature
until oily paste formed and kept at cool dry place for
further use.