Minimum inhibitory concentration (MIC) of ethyl acetate: In a ask, 50 ml of nutrient broth was poured, and in the other ask, 25 ml of nutrient broth was added with 0.625 g from ethyl acetate 40% dry extract to obtain on 25 mg/ml concentra- tion in this ask from extraction.
26 glass tubes were taken and labelled them with numbers from 0 to 25. On each tube, the extract from P. granatum with ethyl acetate 40% concentration which is equal to the number of the label of the tube was taken. For example: in tube number 25, 2 ml from ask 2 was added, which represents 25 mg/ml concentration. In tube number 24, 1.92 ml from ask 2 and 0.08 ml from ask 1 were added, which represents 24 mg/ml concentration, so that with other tubes, in the tube number 0 put only 2 ml from ask 1 to obtain of 0 mg/ml in this tube. e distribution between ask 1 and 2 in these tubes is according to the law C1 V1 = C2 V2.