Probiotics are well known as safety microorganisms that are capable of maintaining, adjusting body balance and improving the immune system of the host organism. They also help in decreasing of cholesterol in blood and promoting of the digestion system. Bifidobacterium spp. are line in lactic acid bacteria group, which are the essential probiotic of human and animals. However, Bifidobacterium group cannot growth in the condition of oxygen present, weak in environmental conditions such as pH so that those results in a low viability compare to other probiotic species