and anterior cingulate cortices. Upon assay, tissue was homogenized with
motor driven pestle in RIPA buffer solution with 1% protease inhibitor
(Calbiochem protease inhibitor cocktail set iii, EDTA free)interestingly in the cerebellum of early and late-stage PD. Importantly, there was no decrease in enzyme levels in the cortex, putamen, or cerebellum of AD. Our results suggest that down-regulation of PPP enzymes and a failure to increase antioxidant reserve is an early event in the pathogenesis of sporadic PD.