During this time period, the mixture
(total volume of 450 mL) was continuously stirred with the aid of a
mechanical stirrer (Kika Labortechnik, Staufen, Germany) while the
pH was kept constant, at the desired value (pH), by adding NaOH or
HCl (Sigma Chemical, St. Louis, USA) solution, accordingly.
Following storage at 4 C for 12 h to soften and loosen the germ
structure, the mixture was subjected to intensive agitation with a
four blade blender (Braun ZK100, Kronberg im Taunus, Germany)
for oil body extraction.