Affected seeds rate = Number of seeds infected with
some fungimotal tested seeds x 100%
Affected germplasm rate = Number of samples infected
with some fungi/Total tested accessions x 100%
Affected range is the percentage that all accessions
infected by the same fungus varied to a certain extent.
Agar plate test Wheat seeds for testing were pretreated
with 1 .O% (v/v) sodium hypochlorite for 2 min
and then rinsed thrice with sterile distilled water. The
sterilized seeds were placed in a 9.0 cm-diameter PDA
plate (potato 200 g, glucose 20 g, agar 20 g, distilled
water 1 000 mL). A total of three plates (10 seeds per
plate) were tested. Seeds were incubated at 25°C for
7- 10 d to allow fungal growth and sporulation with an
8-h NUV light per day (McGee 1994; Mew and Misra
1994; Michail et al. 1999; Li et al. 2001).