I was thinking about
the larger cells stuck in the filter stack growing and dividing
while the medium passed through for those short few minutes.
Then for reasons unknown,since I known othing about poul-
try farming,I began visualizing chickens attached all over the
ceiling ofthe room and thinking that the only (living) objects
that would fall from the surface would be eggs.That was it.I
thought that if cells became stuck with in the filter stack while cul-
ture medium passed through,theonly cells that could be released
from the stack would be newborn cells originating from the por-
tion of the adhered cells that was not involved in the ir adherence
I realized that many ofthe dividing rod-shaped cells might not
release progeny a tall,or that some newborn cells might reat-
tach,but in the ideal casein which attachment was permanent,
only newdaughter cells would bereleased,no matter how few.
Experimentally,strong attachment and good flushing with culture medium ought to yield a highly purepopulation of newborn
cells.The best part was that this could be at ruly minimally dis-
turbed synchronous population because the process of preparing
thesynchronouscellssimplyinvolvedcollectingcellsfallingfrom
a surface-bound culture growing under undisturbed conditions