However, in contrast to starch- and sugar-based biomass sources, more complex physical, chemical, or biological processes are needed to prepare lignocellulosic biomasses [7e9]. In particular, saccharification to fermentable sugars is usually carried out by enzyme catalysis because the process would minimize environmental pollution [10,11]. Endoglucanase, exoglucanase, and bglucosidase perform this saccharification process [12]. Diverse bacteria and fungi have cellulolytic enzyme activities. Fungi of the Trichoderma genus are awell-known source of cellulolytic enzymes and some of these enzymes have been developed as commercial products [13]. In particular, Cellic® CTec2 (Novozyme) is a commercial cellulase enzyme mixture that consists of 15e20 enzymes,including Trichoderma reesei RUT-C30 enzymes