Rice seeds cv. Pathum Thanee 1 was planted in 60-cm diameter cement tank containing clay soil which was analyzed chemical properties: (pH= 7, % OM = 2.35 (moderate), aval. P = 88 mg/kg (very high), exch. K = 230 mg/kg (very high)). Only six healthy seedlings pertreatment per replication were selected to use as
representative for data recording.
When rice plant age was 25-30 days after planting, the first chitosan spraying was done, after that chitosan was again sprayed at 7-10 days intervals for four times through crop season.
Chemical fertilizer at the rate as mentioned above was divided to
apply 3 times at 15, 35 and 55 days after planting.
When age of rice plants was 65 days, all rice plants were divided
into 2 sections (inoculation and non inoculation section),conidial suspension (1x105 conidia/ml) and mycelial plugs (Ø 5 mm) of Helminthosporium oryzae, Curvularia lunata and Fusarium moniliformae were inoculated on wound rice leaves of inoculation section, and then water was immediately sprayed all over rice plants and covered by plastic bag for 24 hrs. for maintaining moisture content within canopy, after that plastic bag was withdrawn from
rice plants.
This experiment was carried out at Rajamangala University of Technology Suvarnabhumi during March to June 2012. All data were subjected to ANOVA by MSTAT and treatment mean comparison was done by the use of Least Significant Difference (LSD).
Rice seeds cv. Pathum Thanee 1 was planted in 60-cm diameter cement tank containing clay soil which was analyzed chemical properties: (pH= 7, % OM = 2.35 (moderate), aval. P = 88 mg/kg (very high), exch. K = 230 mg/kg (very high)). Only six healthy seedlings pertreatment per replication were selected to use asrepresentative for data recording. When rice plant age was 25-30 days after planting, the first chitosan spraying was done, after that chitosan was again sprayed at 7-10 days intervals for four times through crop season. Chemical fertilizer at the rate as mentioned above was divided toapply 3 times at 15, 35 and 55 days after planting. When age of rice plants was 65 days, all rice plants were dividedinto 2 sections (inoculation and non inoculation section),conidial suspension (1x105 conidia/ml) and mycelial plugs (Ø 5 mm) of Helminthosporium oryzae, Curvularia lunata and Fusarium moniliformae were inoculated on wound rice leaves of inoculation section, and then water was immediately sprayed all over rice plants and covered by plastic bag for 24 hrs. for maintaining moisture content within canopy, after that plastic bag was withdrawn fromrice plants. This experiment was carried out at Rajamangala University of Technology Suvarnabhumi during March to June 2012. All data were subjected to ANOVA by MSTAT and treatment mean comparison was done by the use of Least Significant Difference (LSD).
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