Virulence of 12 entomopathogenic fungal isolates against Ae.
albopictus larvae was investigated as follows. Conidial suspensions
were adjusted to 1 × 107 conidia ml−1 using 0.03% siloxane solution
in 90-mm-diameter Petri dishes at 10 ml dish−1. Fifteen third-instar larvae
were placed in the dish using a disposable dropper. Dishes were covered
with lids and kept at 25 ± 1 °C and 16:8 (L/D) photoperiod and
examined daily for 8 days. For dosage dependent assay, the conidial concentration
of the selected isolate (M. anisopliae JEF-003)was 10-fold diluted
from1 × 105 to 1× 107 conidia ml−1 andassayedwith samepetri-dish
conditions as described above. This experiment was replicated 3 times.