To observe the effect of greener-synthesized silver nanoparticles on wood-degrading fungi, the agar disc diffusion
method was performed. To accomplish this, wood degrading fungi were inoculated into the middle of potato dextrose agar plates and incubated at 30 °C for 3 days. When the fungal diameter reached 4–5 cm, sterile paper discs loaded with different concentrations of silver nanoparticles (2 and 4 mg) were placed on the periphery of the fungus. These plates were further incubated at 30 °C until the mycelial growth enveloped the control disc containing the turnip leaf extract. The phytopathogenic wood decay fungi tested were Gloeophyllum abietinum, G. trabeum, Chaetomium globosum, and Phanerochaete sordida. To test the viability of fungal mycelium, the mycelium from the zone of inhibition were placed onto the PDA plates and
incubated at 30 °C for 48 h.