Roche Diagnostics PCR reagents were used with following conditions: 20 μL reaction volume contained 1x PCR buffer, 3.5 mM MgCl2, 250 μM of each dNTP, 3 U FastStart Taq Polymerase, 0.5x EvaGreen Fluorescent DNA Stain (Jena Bioscience), 0.125 μM primer (Art2A) or 0.25 μM primer (BovB) and 5 pg of template DNA.