An enriched culture in PHA-producing bacteria was developed using activated sludge from the aerobic tank of the Wastewater Treatment Plant of Patras (Greece) as inoculum. The Wastewater
Treatment Plant (WWTP) was an Enhanced Biological Phosphorus Removal (EBPR) scheme coupled with a Carrousel system (oxidation ditch designed to effectively reduce the nutrients discharged).
The strategy that was followed for the selection andenrichment of activated sludge in
PHA-accumulating bacteria wasthe alternating cycling between limitation of carbon and nitrogen substrates