Fig. 4. The effects of FBS concentration and individual fatty acid supplementation on Δ6
Fads (filled columns) and Elovl5 (cross-hatched columns) gene expression in the SBT-E1
cell line. In Panel A, the cells were incubated under the different conditions for 3 days
and the fatty acids were supplied at a concentration of 10 μM. In Panel B, the cells were
incubated under the different conditions for 24 h and the fatty acids were supplied at a
concentration of 10 μM. In Panel C, the cellsweremaintained in standard culture medium
containing 10% (v/v) FBS. In Panels A and B, the data have been normalised using β-actin
and are expressed relative to the control, arbitrarily set at a value of 1.0. In Panel C, the
transcript abundances forΔ6 Fads and Elovl5 are expressed relative to the transcript abundance
for β-actin. The data are themean±standard error of themean (n=3). Significant
differences between the treatments and the control are indicated by an asterisk.