in different in vivo and in vitro assays, as well as inhibition of hydrolytic enzyme activity and lipid peroxidation [18]. In this regard, the root extract of Wilbrandia ebracteata (Cucurbitaceae), a plant commonly used in Brazil to treat rheumatic disease, was reported to contain several cucurbitacins [19]. There are limited studies on the chemopreventive effect and the mechanism of action of CLG. Therefore, the present study attempts to understand the inhibitory effects of CLG on reactive nitrogen and oxygen species as an explanation for the apoptogenic effects of this natural cucurbitacin in colon cancer cells (HT-29). Based on our studies, the anti-inflammatory and antioxidant properties of CLG may be regarded as a key attribute for its role against colon tumorigenesis.