2.4. Calculations
Nitrogen-free extract (NFE) was calculated as DM less the sum of
crude protein, crude lipid, and ash. The apparent digestibility coefficients
(ADCs, %) of dietary nutrients and minerals, as obtained from
the direct and total collection method of measuring in experiment
1, were calculated as (Jobling, 1994):
ADCi ¼ 100TðCi−FiÞ=Ci;
where i corresponds to a dietarymacronutrient or mineral (i.e., protein,
lipid, NFE, ash, TP or phytate-P), C is the consumed amount of i, and F is
the fecal loss of i.
Complete N and P mass-balances were set up based on the total
duration of the first experiment (24 days), and following the approach
by Cho et al. (1994) modified to measure dissolved waste directly: