The samples analyzed from different muscles of seafood species used in sashimi from Japanese restaurants presented levels of As, Cr, Hg and Pb above the maximum levels allowed by Mercosul and European regulations. However, variable concentrations were observed during a one-year period suggesting higher concentrations in autumn and winter. The estimated weekly intake shows that tuna, amberjack and octopus sashimi were the highest contributors for As, mullet and amberjack for Pb; tuna and black anchovy for Hg and octopus for Cd and Cr intake.