Listeria species are ubiquitous and has been isolated from poultry, animals, different types of foods, and environments worldwide [9]. Among the differentspecies of the genus Listeria, Listeria monocytogenes is the causative agent of listeriosis [27]. Although poultry flocks are rarely reported as a vector of Listeria outbreaks, the relatively high prevalence of L. monocytogenes in chicken farms presents a potential risk. In Egypt, Some studies already investigated the prevalence of L. monocytogenes in chicken carcasses or fresh chicken meat [1, 2]. However, scanty data are available on Listeria spp prevalence including L. monocytogenesin chicken farm samples especially environmental samples such as, litter, drinking water, and poultry feed.