Four antimicrobial feeding treatments(ampicillin, kanamycin, metronidazole, and tetracycline) were used to experimentally induce dysbiosis in the termite gut and quantify changes community ecology and digestion. To first determine if these antimicrobials could reduce bacterial populations, gut bacteria were isolated and quantified using traditional culturing techniques. The two broad- spectrum antimicrobial kanamycin and tetracycline, significantly reduced the culturable community of bacteria isolated from the termite gutt(Fig. 1A) Ampicillin, which targets gram-positive bacte- ria, and metronidazole an antiprotozoan,did not significantly reduce the culturable bacterial community. Antimicrobial treatments also resulted in non-significant changes to the culturable, anaerobic community(Fig. S1) In addition to reductions in bacterial load, kanamycin, metronidazole, and tetracycline treatments also impacted protist abundance significantly(Fig. IB) overall, all anti- microbial treatments significantly reduced the symbiont load, both bacteria and protists, in a seven-day feeding period(Fig. 1).