Quinone Reductase (QR) Activity
Tissue activities of QR were determined as described by Spencer and Rifkind [43]. For preparation of the homogenates, 30 mg of the frozen tissue was homogenized in 200 μl of 50 mM potassium phosphate buffer, pH 7.6, containing 1mMPMSF,5μg/ml aprotinin, and 5 μg/ml leupeptin.
Statistical Analysis
Statistical analyses were performed with one-way ANOVA using Tukey’s post-hoc test. LDH and liver data were assessed by Student’s t test. A p value less than 0.05 was considered significant.