The shrimp (Metapenaeus monoceros) shells were obtained in fresh condition
from a shrimp processing plant located in Sfax, Tunisia. Shell waste were washed
thoroughly with tap water, mixed with distilled water at a ratio of 1:2 (w/v) and
then cooked for 20 min at 90 ◦C. The cooked sample was drained and homogenized
in a Moulinex® blender for about 2 min then used for moisture determination and
kept at −20 ◦C until further use