Wastewater was obtained from a bleach Kraft mill located in the Campinas city region (São Paulo
State, Brazil). This mill process uses only Eucalyptus grandis wood. The sample effluent was obtained
after the first alkaline extraction stage (E1). The bleaching process utilizes Cl2, oxygen and hydrogenperoxide
reinforced alkaline extraction stage, NaClO4 and hydrogen peroxide. The samples were taken
periodically (approximately once a month) over a three year period. The samples was stored at 4 0C in
bottle flasks and used without previous filtration.