A B S T R A C T
In the course of an in-house validation of an RNA/DNA co-extraction strategy at least two EDNAP
(European DNA Profiling Group) mRNA markers per body fluid were combined into a 12plex. The 12plex
should serve as a screening tool, which supplements DNA and presumptive test results. Based on the
12plex results body fluid specific EDNAP multiplexes were used for further confirmation. From the
validation and the application of this method to challenging forensic casework samples we present the
positive and negative experiences.