(4) The actual temperature at which freezing
is initiated in the plants being studied should be monitored, along
with ice propagation, by infrared video thermography if posssible.
This will enable the identification not only of the freezing tem-
perature but also the determination of whether or not decreased
freeze injury in transgenic plants is the result of the formation of
less ice, variation in the site of formation, rate of ice propagation,
etc. (5) Care should be taken to use well described, realistic, and
multiple freezing regimes to test the resistance of plants.