Materials and methods
Raw Material and dry starter cakes
Glutinous rice RD6 was used as raw material for sato fermentation. Dry
commercial starter cakes were kindly received from Sangpech (Surachae)
Ltd., Surin province.
Isolation of yeasts and fungi from dry starter cakes
One gram of the dry starter cakes were ground and suspended with 9 ml of
sterilized distilled water. One ml of the cell suspension was inoculated to
50 ml of the following medium (g/l, YM medium) in 250 ml Erlenmeyer
flask, glucose 10; peptone 5; malt extract 3; yeast extract 3. After shaking
at 180 rpm at room temperature for 3 days, yeasts and fungi were isolated