Cell culture of the virus in the presence of aqueous extracts
in different sets of experiments was assessed by hemagglutination
assay. The inhibitory effect of the extract is
shown by reducing HA titre (Fig. 5). Virus inhibition
activity of the aqueous extracts showed 100 % inhibition of
the virus in all three experiments at a concentration of 25
and 5 mg/mL except post-penetration exposure analysis
which showed 93.75 % inhibition at 5 mg/mL. There was
[50 % antiviral activity seen with all experiments at
concentration of 2.5 mg/mL. However, when the concentration
was lowered; it has been found that post-penetration
and simultaneous exposure showed 75 % inhibition of the
virus at concentration ranging from 1 to 0.025 mg/mL.
There has been no antiviral activity seen with pre-penetration
exposure analysis with these lower concentrations