Water sample from freshwater bodies, cooling water deposits, soil and sludge were enriched in CHU- no-3 medium. Flask were incubated at ambient temperature for 10 days in chamber with artificial light (white)source. Four serial subculture were made in the fresh medium and algal biomass developed was cultivated under same conditions. Biomass after serial subculture was isolate on CHU-no-3 solid media plate and incubated for 7 days in chamber with artificial light sources.Seleted microalgal species were cultivated in various culture media like Beneck’s liquid medium, CHU no 10, Kuhl and Lorenzen medium, Erddekokt and Salze, Pringsheim medium and Czurda medium [13][14]. Strains were identified using up to genus level using microscopic techniques and Stain C-1 was identified using molecular methods using genetic markers by outside laboratory.