Inflammation is a response to something that was harmful to the cells in the body, But if there is too much work will affect the body as well. For example Alzheimer's disease, atherosclerosis, rheumatoid. The invented medicine to prevent work too. Reverse current drug made from synthetic,If you have received a large amount of side effects on the body as well. The current studies have herbs that have fewer side effects than synthetic. And from the wisdom of the villagers found that boiled water, Phyllanthus fraternus, Phyllanthus acidus and Pandanus amaryllifolius can inhibit inflammation, the researcher also interested to study the effect of herbal soup (Phyllanthus fraternus, Phyllanthus acidus and Pandanus amaryllifolius) on the enzyme inhibition : inducible nitric oxide synthase in vitro cell cultured macrophages RAW 264.7 is activated by lipopolysaccharidecholesterol. The study of cytotoxicity of the water boil herbs with trypan blue exclusion method and Enzyme inhibition test : inducible nitric oxide synthase, by double antibody Sandwich ELISA. Study results showed that the water boil herbs at all concentrations do not affect the viability of the cells, The mixed water boil herbs and boiled herbs Phyllanthus acidus For all concentration, and boiled herbs from Phyllanthus fraternus at a final concentration of 400 μg / ml can inhibit the expression of enzymes inducible nitric oxide synthase get statistical significant (p < 0.05). The effect of inhibition of enzyme inducible nitric oxide synthase, It may be caused by flavonoids in plants. So the ability to launch the curb as possible. Should have studied the ratio of medicinal herbs in boiling water for inhibiting the expression of enzymes inducible nitric oxide synthase into the future.