The main antifungal activity was found in the fraction eluted with the 100% methanol. This fraction was subsequently analyzed by LC–ESI-MS. The total ion count chromatogram (TIC) ion positive mode revealed the presence of two major peaks at 13.51 and 13.93 min. MS data of the first peak (13.51 min) showed two pseudo-molecular ion peaks at m/z 1022 [M+H]+ and 1044 [M+Na]+, which were attributed to the C14 homolog of surfactin. The second peak (13.93 min) from TIC chromatogram displayed both protonated and sodiated ion peaks in the MS spectrum at m/z 1036 [M+H]+ and 1058 [M+Na]+, which were attributed to the C15 homolog of surfactin. Attribution of identity was based on comparison with the authentic standard as well as the literature ( Pecci et al., 2010). LC–ESI-MS data did not show any evidence of the fengycin or iturin family of antimicrobial compounds.