DNA pyrosequencing
Pyrosequencing is a next generation high throughput
DNA sequencing technique that has allowed simultaneous
sequencing of multiple samples (Langaee and
Ronaghi 2005; Parameswaran et al. 2007). Based on
the principle of sequencing by synthesis, it involves
real-time monitoring of DNA synthesis by bioluminescence
using a cascade that upon nucleotide incorporation
ends in a detectable bioluminescence. When a
nucleotide is introduced in the DNA strand, the pyrophosphate
release is quantified. DNA pyrosequencing
has been successfully employed in a variety of applications,
including genotyping, single-nucleotide polymorphism
detection, microbial ecology, and environmental
microbiology (Ahmadian et al. 2006; Brulc et al. 2009;
Li et al. 2012).