The composition of hemp seed oil obtained with supercritical CO2 was compared with the oil extracted by n-hexane using Soxhlet apparatus and with oil obtained by pressing using screwex peller. Table 6 show fatty acids, tocopherol and pigment content of hemp seed oil obtained using different extraction methods where it can be concluded that fatty acid content of hemp oil obtained by all three different extraction methods were very similar, while obtained hemp oil by supercritical CO2 had higher tocopherol content compared to cold pressing. Oil obtained by organic solvent did not have tocopherols according to high temperatures during Soxhlet extraction process. It can be seen that the hemp oil obtained by supercritical CO2 has the lowest total pigment content followed by oil obtained with method by Soxhlet while oil obtained by cold pressing have the highest total pigment content. The highest pigment content obtained by cold pressing can be attributed to the intensive mechanical destruction of hemp seed cells and destruction of chloroplasts (Hannides et al., 2014).