2.1. Aqueous extraction of rice bran oil
RBO was extracted from raw rice bran using distilled water as
the solvent. Rice bran and water were mixed in a container
to the required bran to water ratio and the mixture was continuously
stirred for a specific time at constant temperature
and pH. The sample was then centrifuged to separate cream
(or emulsion), supernatant and solid phase (or defatted bran).
The cream collected was stored over night in the refrigerator.
The cream was kept at room temperature before further processing.
Then the contentwas boiled gently to break emulsion
until oil appeared. Separated upper layer of oil and cream was
washed with warm distilled water and then allowed to settle.
Aqueous phase (clear and oil free) was discarded, and the oil
was decanted. Then the oil was kept in hot air oven for 2h at
105 ◦C until constant weight was obtained. Aqueous extracted
oil was named AERBO