Red sea bream, Pagrus major, is one of the most important fish cultured in Japan. Two clones of
red sea bream were produced. Eggs from a mitotic gynogenetic diploid (mitotic-G2N) red sea bream
were inseminated either with sperm from a mitotic-G2N male to produce a heterozygous clone
(hetero-clone), or with UV-irradiated sperm of Japanese parrot fish (Oplegnathus fasciatus) and the
second meiotic division suppressed by cold shock to produce a homozygous clone (homo-clone).
Normal diploids were also produced from one male and female as a control. The clonal status of the
fish was confirmed by multilocus DNA fingerprinting. The fingerprinting patterns differed between
individuals within the normal diploids. However, there was no variation between individuals within
hetero- or homo-clones. The patterns of the homo-clones and the mother were identical, and all the
bands of homo-clones were also observed in hetero-clones. Thus, the clonal status of homo- and
hetero-clones was confirmed and the production of clones from the broodstock of mitotic-G2N was
achieved. The hatching rates, survival rates and growth of the hetero- and homo-clones were
recorded for a brief comparison with results of diploid controls. D 2002 Elsevier Science B.V. All
rights reserved.
Red sea bream, Pagrus major, is one of the most important fish cultured in Japan. Two clones ofred sea bream were produced. Eggs from a mitotic gynogenetic diploid (mitotic-G2N) red sea breamwere inseminated either with sperm from a mitotic-G2N male to produce a heterozygous clone(hetero-clone), or with UV-irradiated sperm of Japanese parrot fish (Oplegnathus fasciatus) and thesecond meiotic division suppressed by cold shock to produce a homozygous clone (homo-clone).Normal diploids were also produced from one male and female as a control. The clonal status of thefish was confirmed by multilocus DNA fingerprinting. The fingerprinting patterns differed betweenindividuals within the normal diploids. However, there was no variation between individuals withinhetero- or homo-clones. The patterns of the homo-clones and the mother were identical, and all thebands of homo-clones were also observed in hetero-clones. Thus, the clonal status of homo- andhetero-clones was confirmed and the production of clones from the broodstock of mitotic-G2N wasachieved. The hatching rates, survival rates and growth of the hetero- and homo-clones wererecorded for a brief comparison with results of diploid controls. D 2002 Elsevier Science B.V. Allrights reserved.
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