CpFBP1 transformants accumulated increased levels of
CrFBPase protein and extracts contained ~1.4-fold-increased levels of FBPase enzyme activity compared to the
wild type strain. Surprisingly, we found that under photoautotrophic growth conditions and/or with elevated
CO2 levels, this 40% elevation in FBPase activity had a negative effect overall on growth rate and biomass production.
These results suggest that unlike the situation in higher plants, FBPase probably does not catalyze a ratelimiting
step in the C. reinhardtii Calvin cycle