Dehydrated tryptone soy agar media (Oxiod) was used for routine culture of the bacteria under test. The isolation plates of actinomycetes contaminated with bacteria and fungi were purified by streak-plate technique. A small portionof typical isolated colonies were streaked on tryptone-soy agar media or starch casein agar media and incubated at 25°C for 2-7 days (Shellab). Plates were checked for the growth of typical actionmycetes colonies up to 10 days.