The Adams medium favored the Aspergillus sp. GM4
tannase production and is a low cost medium compared
to the other tested culture media. Aspergillus sp. GM4
produces tannase in the presence of tannic acid and methyl
gallate inducers. Gallic acid as the sole carbon
source induced tannase production. This result suggests
that tannase production by Aspergillus sp. GM4 can continue
in the presence of increasing product in the culture
medium and that the end product does not regulate enzyme
production. In addition, the Plackett-Burman and
Central Composite Rotatable designs were shown to be
effective in reducing the number of components in the
medium composition and increased tannase production
2.66-fold.