MATERIALS AND METHODS
Enzymolytic soybean meal Two soybean meal products, extracted dehulled soybean meal (SBM) and its hydrolysates with microbial protease, enzymolytic soybean meal (ESBM), were obtained from Continent Biotech (Qingdao, China). The process of ESBM product included SBM fermented (1:2 wt/vol in distilled water) at pH 4.5 for 12 h, then incubated with derivatives thereof, microbial protease, for 24 h at 50°C and dried at 65°C.
Diets and animals The control diet was based on corn-soybean meal and experimental diets contained ESBM at 5, 10 or 15% inclusions in the basal diet. During the trial, no antibiotics were offered to weaned pigs via either feed or water. The formulation of the diets is given in Table 1. A total of 240 weaned piglets (Duroc×Landrace×
Yorkshire, 28 days old) with similar body weight (9.01±0.22 kg body weight) were randomly allocated to four treatments with 6 pens per treatment and 10 piglets per pen. The temperature of the pig shed was 20 to 22°C and the relative humidity was 50 to 70%. The pigs were fed ad lib. and had free access to water. The body weight and feed intake of pigs were measured weekly on pen basis. Diarrhea was monitored daily throughout the experiment. This trial was conducted for 28 days in accordance with laws and regulations that control experiments and procedures in live animals in China, as overseen by the Chinese Animal Research Authority.
MATERIALS AND METHODS
Enzymolytic soybean meal Two soybean meal products, extracted dehulled soybean meal (SBM) and its hydrolysates with microbial protease, enzymolytic soybean meal (ESBM), were obtained from Continent Biotech (Qingdao, China). The process of ESBM product included SBM fermented (1:2 wt/vol in distilled water) at pH 4.5 for 12 h, then incubated with derivatives thereof, microbial protease, for 24 h at 50°C and dried at 65°C.
Diets and animals The control diet was based on corn-soybean meal and experimental diets contained ESBM at 5, 10 or 15% inclusions in the basal diet. During the trial, no antibiotics were offered to weaned pigs via either feed or water. The formulation of the diets is given in Table 1. A total of 240 weaned piglets (Duroc×Landrace×
Yorkshire, 28 days old) with similar body weight (9.01±0.22 kg body weight) were randomly allocated to four treatments with 6 pens per treatment and 10 piglets per pen. The temperature of the pig shed was 20 to 22°C and the relative humidity was 50 to 70%. The pigs were fed ad lib. and had free access to water. The body weight and feed intake of pigs were measured weekly on pen basis. Diarrhea was monitored daily throughout the experiment. This trial was conducted for 28 days in accordance with laws and regulations that control experiments and procedures in live animals in China, as overseen by the Chinese Animal Research Authority.
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