1) Topoisomerase removes the helical shape.
2) Helicase separates the two DNA strands.
3) Binding proteins attach to prevent the strands from rejoining.
4) Leading strand is 5’ 3’; here DNA polymerase III forms a new strand continuously.
5) Lagging strand is 3’ 5’ but it has to form 5’ 3. Here DNA formation is discontinuous.
6) This is done in Okazaki fragments.
7) RNA Primase applies RNA primer
8) RNA Polymerase III makes new DNA.
9) Process repeats many times.
10) DNA Polymerase I replaces the RNA primer with DNA.
11) Finally, DNA ligase links the Okazaki fragments.