Genomic DNA prepared in agarose plugs was digested with
S1-nuclease, separated by PFGE, and again hybridised with 32P
radio-labelled blaCTX-M-14 DNA. Subsequent analysis allowed
identification of chromosomal and/or plasmidic locations of the
blaCTX-M-14 genes. Replicon types of plasmids carrying blaCTX-M-14
were deduced by multiplex PCR assay (Carattoli et al., 2005).