ene.
In silico analysis of the genomic region in the vicinity of
Xa33 for putatively expressed genes. Based on the fine mapping
analysis, the SSR marker RMWR7.1 was located at a genetic
distance of 0.9 cM on one side of the gene and RMWR7.6 was
located on the other side of the gene at a genetic distance of
1.2 cM (Fig. 1). The intervening genomic region, consisting of
≈49 kb of sequence, was downloaded from the Japonica genome
sequence database (http://rise.genomics.org.cn) and analyzed for
the presence of putative genes using the software FGENESH
(http://www.softberry.com). The functionality of the genes was
analyzed using the BLAST-P tool (1), available online at http://
www.ncbi.nlm.nih.gov. Eight genes were observed to be present
in the genomic region flanked by RMWR7.1 and RMWR7.6. Of
the eight genes identified in the region, one gene encoding serine
threonine kinase, one encoding putative transposon protein and
mutator protein, one encoding ribose-5-phosphate isomerase
subfamily, one encoding domain of unknown function 295, one
encoding DNA repair/transcription protein Mms-19-like protein,
two encoding base inhibitor 1 (which belongs to a protein superfamily), and one encoding a hypothetical protein with no putative
conserved domain were detected. The details of the genes along
with their size in base pairs, amino acids, and their putative
functions are given in Table 3