. The present study describes the development of an efficient and reliable system for the plantlet regeneration of a selected genotype of aloe (sweet A. vera genotype) from inflorescence axis-derived callus cultures. Among the different explant materials which were evaluated for the establishment of the callus cultures, the soft base of young inflorescences axis (Fig. 1b) was determined to be the most productive explant source [10]. Older plant bases did not respond to the culture system, while segments of mature inflorescence produced watery, glossy, and non-regenerative callus cultures. Other explants, i.e., axillary buds, roots, and root tips responded also very poorly. Moreover, pre- treatment with a combination of bavistin and streptomycin (0.1% aqueous solution of each) for 15 min and subsequent surface sterilization with 0.1% mercuric chloride for 3 min sterilized the explants and prevented fungal and bacterial growth in cultures. The microbe- free cultures have been maintained in laboratory for more than 3 years. Treatment of explants before and after surface sterilization with chilled and sterile anti-oxidant solution prevented the leaching of phenolic substances from cut ends of the explants thus prevented browning of medium and tissues in the culture system.