Control of Fusarium Wilt of Chili Seeds. Similar
preparation as mentioned previously was done for this
examination, except that the chili seeds were soaked with
2-days old culture (≈ 106 cell/ml) of each chitinolytic
bacterial isolate for 30 min. The experiment was repeated
4 times. Controls were isolate-free seeds planted in fungusfree
soil (IFFF) and isolate-free seeds planted in fungusinoculated
soil (IFF).
Observations of plant height and number of leaves
were conducted every week by randomly chosen three
seedlings for each treatment. Dry-weight was measured
at end of study. Number of seedling damping-off were
observed from total seedlings and damping off reduction
(%) was measured as:
[(number of IFF damping off - number of seed treated
damping off ) / (number of IFFF seedlings)] x 100%