Modification of incubation temperature and inoculum size
A lower incubation temperature of 37°C favours the growth of bifidobacteria (Kneifel et al., 1993).
Using a high level of inoculum, will ensure a high cell count at the end of the incubation and survival of the probiotic bacteria during storage until consumption (Samona & Robinson, 1994). An inoculum level of 10–20% is recommended by Varnam and Sutherland (1994). Rasic and Kurmann (1983) recommended the use of a freeze-dried DVS culture. Concentrated starter cultures (liquid, frozen or dried), should contain a minimum of 5×109 cfu g−1, and unconcentrated starter cultures a population of 1×108 cfu g−1 (IDF, International Standard 149, 1996).
Modification of incubation temperature and inoculum sizeA lower incubation temperature of 37°C favours the growth of bifidobacteria (Kneifel et al., 1993).Using a high level of inoculum, will ensure a high cell count at the end of the incubation and survival of the probiotic bacteria during storage until consumption (Samona & Robinson, 1994). An inoculum level of 10–20% is recommended by Varnam and Sutherland (1994). Rasic and Kurmann (1983) recommended the use of a freeze-dried DVS culture. Concentrated starter cultures (liquid, frozen or dried), should contain a minimum of 5×109 cfu g−1, and unconcentrated starter cultures a population of 1×108 cfu g−1 (IDF, International Standard 149, 1996).
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